Thermo FS Sl 16

PRODUCT DESCRIPTION

Multifunctional centrifuges are characterized by a wide variety of rotors and adapters that fit into one centrifuge. Multifunctional centrifuges can perform a variety of separation procedures, such as microplates, tubes and transfusion bags, and perform clinical protocols, sample preparation and cell culture using a minimum of laboratory space.
Multifunctional centrifuges allow centrifuging samples from 0.2 ml to 4 l in various containers: Eppendorf tubes for 0.2-2 ml, 5 ml; 15 ml or 50 ml Falcon type tubes; striped tubes for PCR; glass or polyalomer tubes (from 175 µl to 600 ml) with or without a lid, with a flat, round or conical bottom, ELISA plates, PCR plates.
When choosing a rotor, you can opt for models with an aerosol-tight lid, which provide complete microbiological safety. Lids are available in opaque and transparent, allowing you to control the centrifugation process and increase the safety and convenience of working with samples.

For medical institutions, models of multifunctional centrifuges with a full load of 24 tubes of 5 and 7 ml (vacutainers), with a full load of 30 tubes of 1.5 and 2 ml, or with a full load of 196 tubes of 5 and 7 ml (vacutainers) will be of interest.

Centrifugation is the process of separating particles of different density and aggregate state into fractions under the action of centrifugal force. The separation speed depends on the centrifugal acceleration (g), which is directly proportional to the angular velocity of the rotor and the distance between the particle and the axis of rotation.

For high-quality centrifugation, the important parameters are the speed of acceleration to the set speed and the speed of deceleration at the end of centrifugation. Soft start allows you to carefully separate (fractionate) samples, such as blood, without the risk of damaging their components. On the other hand, fast acceleration to the set speed in 15-30 seconds and smooth braking, on the other hand, make it possible to make the separation of the fractions as complete as possible, as well as to prevent mixing of the fractions during the stop of the rotor. This method is well established for the isolation of subcellular particles and viruses from biomaterial.

The basic rule of operation on a centrifuge is to place an even number of test tubes in the rotor, one opposite the other of equal volume. This approach ensures uniform distribution relative to the axis of rotation and extends the life of the rotor.

Refrigerated multifunctional centrifuges are ideal for a wide range of applications, especially temperature sensitive samples, and are capable of maintaining temperatures between -10°C and +40°C. In addition to keeping samples safe, the cooling function prevents the equipment from overheating during long centrifugation runs.

Multifunctional centrifuges are equipped with various rotors. The choice of a rotor or rotors must be approached with all due attention in order to make the most full use of all the possibilities offered for the implementation of the tasks. There are horizontal (bucket-rotors), angular, vertical, almost vertical and drum rotors.

Bucket rotors (horizontal rotors) are rotors with oscillating cups and a variable angle. In such rotors there are hinged nests for glasses. Test tubes (with or without adapters) are placed in beakers, which, during centrifugation, take a horizontal position at 90 degrees to the axis of rotation. Precipitation occurs strictly at the bottom of the tube, which simplifies the handling and storage of samples. Bucket rotors provide high load capacity and are used to centrifuge both small and large volume samples up to 4 L, allowing full volume tubes to be centrifuged. Bucket rotors are widely used in medical and research laboratories when low speeds up to 6,000 rpm or accelerations up to 5,590 g are sufficient.

Angle rotors are rotors with a fixed angle of rotation, usually at 40-45 degrees. Angle rotors give the highest speed up to 18,000 rpm and acceleration up to 31,150 g, therefore they are used to precipitate light fractions in a volume of up to 400 ml and reduce centrifugation time compared to bucket rotors.

Vertical rotors are characterized by the fastest centrifugation due to the fact that the particles run a short distance and, accordingly, are deposited faster. During rotation, the test tubes in such rotors are arranged vertically. Sedimentation occurs along the entire far wall of the test tube from the axis of rotation. Therefore, vertical rotors are not suitable for the classical method where localized sedimentation is desired. These rotors are used for density gradient separation, such as DNA or viral particles, and sedimentation on a liquid bed.

Almost vertical (near vertical) rotors differ in the arrangement of test tubes during centrifugation at an angle of 7.5-9 degrees to the axis of rotation. Due to this, the particles overcome a small distance and, accordingly, are deposited faster. Unlike vertical rotors, the presence of a small angle causes the particles to settle to the bottom of the tube, and not along the entire wall. The main advantage of this method is that the precipitated particles do not come into contact with the solution as a result of centrifugation.

Drum rotors (drum-rotors) allow you to get speeds up to 14,000 rpm and acceleration up to 16,435g with a horizontal arrangement of test tubes. For example, in similar bucket rotors with a similar horizontal arrangement of test tubes, the maximum possible speed is only 6,000 rpm. Another advantage of drum rotors is good capacity – up to 60 tubes of 1.5 or 2.0 ml. During centrifugation, the precipitate is concentrated strictly at the bottom of the tube. The formed precipitate must be dense so that it does not crumble when the tube is removed. Such rotors have proven themselves to be useful for isolating nucleic acids from a large number of samples.

Accessories: rotors, cups, lids, inserts, adapters.

Centrifugation is the separation of particles of different density and aggregate state into fractions under the action of centrifugal force. The separation speed depends on the centrifugal acceleration (g), which is directly proportional to the angular velocity of the rotor and the distance between the particle and the axis of rotation, so it is important to relate the diameter of the rotor to its rotational speed. In order to convert relative units of speed (rpm, rpm) into absolute ones (rcf, g), it is necessary to refer to the Dole-Kotzias nomogram in Diaem reference materials or automatic conversion, where, by setting the values ​​of rotation speed and rotor diameter, you can determine the value acceleration g.

Depending on the functionality, centrifugation volumes and rotation speeds, you can choose the right model:

• microcentrifuges – for test tubes of the Eppendorf type, not more than 2 ml;
• minicentrifuges – maximum rotor capacity 400 ml;
• high-performance – for 4, 6 and 9 liters;
• multifunctional high-speed – maximum load capacity of 3 liters and a wide selection of rotors;
• ultracentrifuges – up to 1,019,000 g;
• flow – for the separation of liquids in a continuous or semi-continuous process.

Specialized laboratory centrifuges are vortex centrifuges which provide centrifugation and shaking, hematocrit centrifuges – centrifugation of hematocrit tubes to determine the hematocrit number, erythrocyte volume fraction, cytological – to create a monolayer of cells on a glass slide; centrifuge-evaporators – for concentrating samples by evaporating solvents under vacuum at elevated temperature.